Development and Validation of Stability indicating RP-HPLC Method for the estimation of Azithromycin Suspension

A RP-HPLC method was developed and validated for quantitative determination of azithromycin in pharmaceutical suspension dosage forms. The chromatography was carried out on a Phenomenex C 18 (150 x 4.6 mm i.d., 5μ) column with Acetonitrile: 0.5 % Formic acid as mobile phase (Isocratic A: B = 40: 60 % v/v), at 215 nm detector wave length with a flow rate of 1 ml/min. Clarithromycin was used as an internal standard. The linearity was established in the range of 20 600 ng/ml for HPLC. The HPLC method was accurate and precised for azithromycin suspension with a recovery of 98.75 to 99.44%. The spiked sample solutions were stable upto 1 month. This validated method can be used for estimation of azithromycin in pharmaceutical suspension.